Calculated myofibrillar Ksyn (a), myofibrillar Kdeg (b), cytosolic Ksyn (c), and cytosolic Kdeg (d), RNA concentration (e), total RNA (f), RNA Ksyn (g), RNA Kdeg (h), wet weight (i), wet weight to body weight ratio (j), and mean fiber CSA (k) percent (%) difference from weight bearing (WB) from gastrocnemius muscles of HS (n=8) and HS massaged limb, HSM (n=9). Also, body weight percent difference from WB in HS and HSM animals (l), respectively. Values are mean ± SEM. Independent samples t-tests were used to determine statistical significance between HS and HSM: #different from HS, p < 0.05.