Table 3. Western blot analysis of intracellular signaling pathways in massaged limb (WBM or HSM) and contralateral non-massaged limb (WBM-L or HSM-L) in response to massage.
Total and phosphorylated analysis of signaling proteins from WB (n=8), WB massaged limb WBM (n=9), HS (n=8), HS massaged limb HSM (n=9), WB non-massaged contralateral limb WBM-L (n=9), and HS non-massaged contralateral limb HSM-L (n=9). All values are in arbitrary density units. Values are mean ± SEM. Two-tailed independent samples t-tests between the respective control (WB or HS) and either the massaged limb (WBM or HSM) or contralateral non-massaged limb (WBM-L or HSM-L) were used to determine statistical significance: *different from WB, #different from HS, p < 0.05. Representative images for the data within this table are in Supplemental Figure 5.
| WB | WBM | HS | HSM | WBM-L | HSM-L | ||
|---|---|---|---|---|---|---|---|
| Total Akt | 0.90 ± 0.06 | 0.80 ± 0.04 | 0.76 ± 1.00 | 0.96 ± 0.05 | 0.62 ± 0.04* | 0.86 ± 0.06 | |
| Phospho-Akt | 0.86 ± 0.05 | 0.98 ± 0.08 | 0.90 ± 0.11 | 0.99 ± 0.13 | 0.83 ± 0.08 | 0.80 ± 0.08 | |
| Total FOXO3A | 0.92 ± 0.08 | 0.90 ± 0.07 | 0.92 ± 0.10 | 0.99 ± 0.07 | 1.02 ± 0.07 | 1.05 ± 0.08 | |
| Phospho-FOXO3A | 0.83 ± 0.05 | 0.78 ± 0.05 | 0.85 ± 0.04 | 0.83 ± 0.04 | 0.73 ± 0.04 | 0.82 ± 0.07 | |
| Total FAK | 2.70 ± 0.31 | 3.80 ± 0.55 | 3.70 ± 0.26 | 4.20 ± 0.44 | 3.80 ± 0.39 | 4.20 ± 0.26 | |
| Phospho-FAK | 0.80 ± 0.14 | 0.96 ± 0.08 | 0.92 ± 0.09 | 0.88 ± 0.08 | 0.82 ± 0.10 | 0.89 ± 0.06 | |
| Total ERK1/2 | 0.84 ± 0.07 | 0.87 ± 0.03 | 0.82 ± 0.06 | 0.89 ± 0.05 | 0.86 ± 0.04 | 0.88 ± 0.05 | |
| Phospho-ERK1/2 | 0.91 ± 0.04 | 0.89 ± 0.04 | 0.95 ± 0.06 | 0.94 ± 0.05 | 0.90 ± 0.05 | 0.95 ± 0.06 | |
| Total eEF2 | 0.85 ± 0.06 | 0.85 ± 0.05 | 1.00 ± 0.14 | 0.91 ± 0.10 | 0.90 ± 0.05 | 0.91 ± 0.09 | |
| Phospho-eEF2 | 0.91 ± 0.12 | 0.91 ± 0.07 | 0.98 ± 0.07 | 0.97 ± 0.07 | 0.96 ± 0.09 | 0.96 ± 0.04 | |
| Total S6K1 | 0.83 ± 0.06 | 0.92 ± 0.05 | 0.94 ± 0.03 | 1.05 ± 0.05 | 1.04 ± 0.05* | 0.95 ± 0.06 | |
| Phospho-S6K1 | 0.68 ± 0.13 | 0.83 ± 0.16 | 0.39 ± 0.07 | 0.62 ± 0.16 | 1.00 ± 0.15 | 0.68 ± 0.13 | |
| Total 4EBP1 | 0.79 ± 0.05 | 0.83 ± 0.05 | 1.00 ± 0.08 | 1.10 ± 0.06 | 0.89 ± 0.04 | 1.10 ± 0.05 | |
| Phospho-4EBP1 | 0.88 ± 0.08 | 0.82 ± 0.09 | 1.10 ± 0.08 | 1.10 ± 0.12 | 0.93 ± 0.10 | 1.30 ± 0.07 | |
| Total rpS6 | 1.07 ± 0.11 | 1.00 ± 0.09 | 1.15 ± 0.12 | 1.10 ± 0.06 | 1.06 ± 0.06 | 1.07 ± 0.07 | |
| Phospho-rpS6 | 0.87 ± 0.16 | 0.73 ± 0.18 | 0.73 ± 0.13 | 0.95 ± 0.23 | 0.94 ± 0.13 | 1.39 ± 0.20# | |
| Total MuRF1 | 0.89 ± 0.07 | 0.94 ± 0.07 | 0.90 ± 0.06 | 0.92 ± 0.05 | 1.02 ± 0.06 | 1.03 ± 0.08 | |
| Total UBF-1 | 1.00 ± 0.11 | 1.20 ± 0.11 | 1.03 ± 0.08 | 1.20 ± 0.11 | 1.40 ± 0.07* | 1.24 ± 0.07 | |
| Phospho UBF-1 | 0.94 ± 0.05 | 0.90 ± 0.08 | 0.84 ± 0.08 | 0.85 ± 0.09 | 0.96 ± 0.05 | 0.91 ± 0.09 | |
| Total c-Myc | 0.73 ± 0.11 | 0.68 ± 0.11 | 0.72 ± 0.05 | 1.04 ± 0.14# | 0.74 ± 0.08 | 0.95 ± 0.09 |
All values are in arbitrary density units. Values are means ± SEM.
Different from WB.
Different from HS.