Skip to main content
. 2020 May 26;117(23):13105–13116. doi: 10.1073/pnas.1917906117

Fig. 6.

Fig. 6.

Rab10 colocalizes with DOPr in Golgi-associated compartments. HEK293 cells transiently transfected with FLAG-DOPr alone (A) or together with GalT-YFP (B) a marker of the Golgi apparatus trans cisternae were fixed and prepared as described in SI Appendix. FLAG-DOPr was labeled with a rabbit polyclonal anti-FLAG antibody and an Alexa Fluor 594 goat anti-rabbit IgG antibody (a, green). Endogenous Rab10 was labeled with a specific mouse monoclonal anti-Rab10 antibody and an Alexa Fluor 647 goat anti-mouse IgG antibody (b, red). GalT-YFP was labeled with a chicken anti-GFP antibody and an Alexa Fluor 488 goat anti-chicken IgY antibody (B, c, blue). In merge panels, colocalization between FLAG-DOPr and Rab10 appears in yellow (A, c, and B, d), and triple colocalization between FLAG-DOPr, Rab10, and GalT-YFP appears in white (B, d). The images shown are single confocal slices and are representative of ∼100 observed cells over three independent experiments. (Scale bars, 10 µm.)