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Expression and synthesis of ACA-01 fragments. (A) Strategy for the generation of ACA-01(17–97) (5) through the expression of a His6-SUMO-ACA-01(17–97) (10) fusion followed by proteolysis with Ulp1. (B) Synthesis of ACA-01(1–15) (sulfo)peptide thioesters fragments (6–9) via Fmoc-strategy SPPS.
