Fig. 5.
Binding of semisynthetic ACA-01 (sulfo)proteins 1–4 to CC chemokines. Affinities (pKd) derived from the competitive fluorescence anisotropy data for binding of all ACA-01 isoforms (1–4) and recombinantly expressed ACA-01 (rACA-01). Each panel shows the binding affinity of all isoforms for one chemokine: CCL7 (A) and CCL26 (B). Data represent the average pKd ± SEM of values from three independent experiments, each recorded in duplicate. *, #, and $ indicate significant differences from unsulfated ACA-01, Tyr10 ACA-01, and Tyr12 ACA-01, respectively. Significance (one-way ANOVA) is shown as ** P < 0.01; ***, ###, $$$ P < 0.001; ****, ####, $$$$ P < 0.0001. See SI Appendix, Figs. S21 and S22 for binding data.