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. 2020 May 20;117(23):13117–13126. doi: 10.1073/pnas.2000430117

Fig. 3.

Fig. 3.

Using DCyFIRplex profiling to recapitulate known agonist interactions. (A) DCyFIRplex profiles for known GPCR agonists in our 30-receptor panel deconvoluted via qPCR. (B) Same samples as in A deconvoluted using NanoString. (A and B) ΔCq values correspond to the Cq difference between treated and untreated conditions, with error bars representing the SEM of n = 6 repeats derived from 3 independent 300-plex consolidations deconvoluted in technical duplicate. ΔCq values correspond to a log2 scale. NanoString transcript counts were collected in technical duplicate, averaged, and normalized to the maximal RNA transcript count for each GPCR gene.