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. 2020 May 20;117(23):13117–13126. doi: 10.1073/pnas.2000430117

Fig. 4.

Fig. 4.

Using DCyFIRplex to discover interactions for known GPCR agonists. (A) DCyFIRplex profiles identifying GPCR–ligand interactions (pink bars) discovered in the process of screening known agonists (purple bars) within our panel of 30 exploratory receptors. (B) DCyFIRscreen profiles confirming the DCyFIRplex discoveries in A, with error bars representing the SD of n = 4 experimental replicates. (C and D) Select titrations confirming the DCyFIRplex discoveries in A, with error bars representing the SD of n = 4 experimental replicates. Dashed lines and boxes indicate datasets showing that KYNA activates HCAR3 with greater potency than GPR35 and is also an endogenous negative allosteric modulator of ADRA2B. Full titration datasets are available in SI Appendix, Fig. S5.