Fig. 3.

Depletion of AhR in CD11c-expressing cells did not affect the number of epidermal LC. The back and ear skin of B6 and CD11c-AhR^−/− mice was removed, epidermal sheets were prepared and incubated with EpCAM-FITC and MHC-II-PE (Right) or CD207-PE (Left) antibodies, followed by DAPI nuclear staining. Image analysis was performed to measure the number of positively stained cells by NIH Image J in five random high-power fields. The B6 and CD11c-AhR^−/− mice displayed similar numbers of LC with their typical dendritic morphology. Representative pictures of one out of two experiments are shown.