Skip to main content

View full-text article in PMC

. 2020 May 26;117(23):12799–12805. doi: 10.1073/pnas.2001325117

Table 1.

Michaelis–Menten kinetic constants for DabA, KabA, and amino acid variants

	GPP			DMAPP			l-Glu
Enzyme	k_cat _app (min⁻¹)	K_m (μM)	k_cat _app/K_m (M⁻¹min⁻¹) × 10⁶	k_cat _app (min⁻¹)	K_m (μM)	k_cat _app/K_m (M⁻¹min⁻¹) × 10⁶	k_cat _app (min⁻¹)	K_m (mM)	k_cat _app/K_m (M⁻¹min⁻¹) × 10³
DabA	4.3 ± 0.1	0.33 ± 0.03	12.9	Non MM	Non MM		4.8 ± 0.3	21 ± 4	0.23
DabA T143M	0.65 ± 0.04	13 ± 2	0.052	1.2 ± 0.1	130 ± 20	0.0092
DabA H436A							6.9 ± 0.6	68 ± 16	0.10
KabA	0.29 ± 0.01	89 ± 10	0.0033	1.3 ± 0.1	1.1 ± 0.2	1.2
KabA M114T	0.51 ± 0.02	0.51 ± 0.06	1	0.28 ± 0.01	2.2 ± 0.3	0.13