Fig. 5.

Spawning success, fecundity (no. of embryos/clutch), fertilization, and survival of embryos produced during zebrafish pairwise spawning following i.p. injections. (A) Percent of spawning success of WT and scg2a^−/−;scg2b^−/− matings. Means of 28 to 40 spawning trials are presented. Significant differences were determined using a one-tailed Fisher’s exact test. Letters (a, b, c, d) above bars signify differences between groups and treatments (P < 0.05). (B) Fecundity or the number of embryos produced per spawning couple. Mean + SEM of 3 to 30 successful spawning events are presented. (C) Mean + SEM % fertilization and (D) % embryo survival of mating crosses (n = 3 to 30). Significant differences were determined using a two-way ANOVA followed by a Holm–Sidak post hoc test. Lines connecting different bars represent the comparison between WT and scg2a^−/−;scg2b^−/− fish.