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SDS-PAGE analysis of HpaA protein expression in BL21 (DE3). Lane M: molecular weight marker (15, 25, 35, 45, 60, 80, 100, 140, 180) ×103; lane 1: induced control strain BL21 (DE3) with 0.5 mM IPTG; lane 2: noninduced HpaA; lane 3: induced HpaA with 0.5 mM IPTG; lanes 4, 5, and 6: affinity-purified HpaA (lane 6 corresponds to peak fraction eluted).
