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. 2020 Jun 5;2020:1216926. doi: 10.1155/2020/1216926

Figure 5.

Figure 5

CXCR7 inhibits endothelial-to-mesenchymal transition (EndMT) by the Wnt/β-catenin pathway in HUVECs. (a) Cell immunofluorescence staining with α-SMA Ab in different treated HUVECs. Scale bar, 50 μm. (b) After cells upregulated or downregulated CXCR7 were treated with SDF-1(100 ng/ml), the protein level of α-SMA was detected by western blot analysis. (c) Western blot analysis showed that the expression of α-SMA decreased by using the Wnt/β-catenin inhibitor (ICG001, 1 μg/ml) in the SDF-1 (100 ng/ml) and CXCR7-siRNA group. si-NC: siRNA negative control group. oe-NC: overexpression negative control group. ∗p < 0.05 versus untreated control group, ∗∗p < 0.01 versus untreated control group, ∗∗∗p < 0.001 versus untreated control group, ###p < 0.001 versus SDF-1 group.