Figure 2.

CCl₄ administration induces intrahepatic ER stress and apoptosis in mice. Male BALB/c mice were randomly grouped and injected with olive oil (control) or CCl₄ (n = 10, total mice = 100). (a) Serum ALT levels determined by the rate method. (b) Liver histology (magnification ×100). Hash sign indicates the necrotic area. (c) Relative protein levels of ER stress and hepatocyte apoptosis in mouse livers at the indicated time points postinjection were determined by Western blot. Representative blots and histology from four independent experiments are shown. Histograms represent mean ± SD of four independent experiments. ^∗P < 0.05, ^∗∗P < 0.01 versus the control group.