Figure 4.

Inhibition of eIF2α dephosphorylation mitigates ER stress in response to CCl₄ injury. (a) Levels of intrahepatic ATF6, XBP1s, and CHOP in control, salubrinal, ISRIB, PBA, and CCl₄ groups. (b) Salubrinal, ISRIB, or (c) the overexpressed DnaJC3 altered the expression of intrahepatic ATF6, XBP1s, and CHOP which was determined by Western blot. (d) Immunohistochemistry staining of CHOP expression in the liver (magnification ×100). Representative blots and immunohistochemistry from four independent experiments are shown. Histograms represent mean ± SD of four independent experiments (n = 8-10). ^∗∗P < 0.01 versus the control group. ^†P < 0.05, ^††P < 0.01 versus the CCl₄ or AAV8+CCl₄ group.