Figure 2.

Influenza Virus M2 and PB1-F2 Proteins Stimulate Oxidized DNA Release

(A–C) HEK293FT cells were transfected with the expression plasmid encoding EGFP or influenza virus proteins in the presence or absence of poly(I:C) (A, B) or influenza viral RNA (C). Cytosolic mtDNA was assessed by quantitative PCR at 24 h post transfection.

(D and E) HEK293FT cells were transfected with the expression plasmid encoding EGFP or influenza virus proteins in the presence or absence of poly(I:C). Pure cytosolic extracts were collected at 24 h post transfection. Oxidized DNA in the cytosol was detected by dot blot analysis using anti-8OH-dG antibody.

(F and G) HEK293FT cells were infected with WT (rgPR8), rgPR8/M2del29–31 (F), or rgPR8/ΔPB1-F2 virus (G). Cytosolic mtDNA was assessed by quantitative PCR at 24 h post infection. These data are from three independent experiments (A–C, F, G; mean ± SEM).

∗∗p < 0.01 and ∗∗∗p < 0.001 versus EGFP-transfected or rgPR8-infected cells (one-way ANOVA and Tukey's test).