Figure 5.

Influenza Virus Stimulates the AIM2 Inflammasome-Mediated IL-1β Secretion

(A–C) LPS-stimulated BMMs prepared from WT or AIM2-deficient mice were infected with HSV-1ΔVP22 (A) or PR8 virus (B) for 24 h. For ATP stimulation, BMMs were stimulated with LPS for 6 h and pulsed with 2 mM ATP for 30 min (C).

(D and E) LPS-stimulated BMMs were infected with PR8 virus for 24 h in the presence or absence of mtDNA fragment prepared as described in Methods (D) or DNase I (E). Cell-free supernatants were collected and analyzed for IL-1β by ELISA. These data are from three independent experiments (mean ± SEM).

∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA and Tukey's test). See also Figure S3.