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Conventional mono-mode IMAC enrichment yields considerable phosphopeptide coverage (A), while less coverage of M6P glycopeptides (B); Dual-mode affinity enrichment approach enables the separation of M6P glycopeptides from phosphopeptides: (C) Workflow of large-scale profile of phosphopeptides and M6P glycopeptides in five mouse tissues; (D) Phosphopeptide identification results from mouse lung; (E) M6P glycopeptide identification results from mouse lung.
