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. 2020 Jun 10;13:5353–5361. doi: 10.2147/OTT.S255675

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© 2020 Tian et al.

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MCTS1 directly bound with TWF1 in luminal A/B breast cancer cells. (A) Proteins with predicted interactions with MCTS1. (B, C) Isoform percentage of the dominant TWF1 transcript (ENST00000395510.6) (B) and its absolute expression (C) in normal breast tissues from GTEx-breast and cancer tissues from TCGA-BRCA. (D and F) Western blot analysis of cyclin D1, C-Myc, MCTS1 and TWF1 expression in BT-474 (D) and MCF-7 (F) cells 48h after lentiviral infection for MCTS1 or TWF1 overexpression. (E and G) CCK-8 assay was performed to examine the proliferation of BT-474 (E) and MCF-7 (G) cells with or without TWF1 overexpression. (H) IF staining of MCTS1 (blue) and TWF1 (red) in BT-474 (up) and MCF-7 (down) cells. (I, J) Co-IP assay was performed to investigate the potential binding between MCTS1 (with DDDDK tag) and TWF1 (with HA tag) in BT-474 (I) and MCF-7 (J) cells. **p<0.01; ***p<0.001. (p≥0.05).

Abbreviation: n.s., not significant.