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. 2020 Jun 10;13:5353–5361. doi: 10.2147/OTT.S255675

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© 2020 Tian et al.

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MCTS1 and TWF1 synergistically modulate cyclin D1 and C-Myc translation. (A, B) QRT-PCR analysis of CCND1 and C-MYC transcription in BT-474 cells 48h after lentivirus-mediated MCTS1 (A) or TWF1 (B) overexpression. (C, D), (F, G) Western blot analysis of MCTS1 and TWF1 expression in BT-474 (C, D) and MCF-7 (F, G) cells 48h after lentivirus-mediated MCTS1 (C and F) or TWF1 (D and G) knockdown. (E and H) Western blot analysis of cyclin D1 and C-Myc expression in BT-474 (E) and MCF-7 (H) cells 48h after lentiviral infection for MCTS1 or TWF1 overexpression alone, or in combination with simultaneous TWF1 or MCTS1 knockdown. (p≥0.05).

Abbreviation: n.s., not significant.