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Generation of mice with Zα2 domain deletion in ZBP1 (Zbp1ΔZα2/ΔZα2). A, schematic of domain structure and deletion of the Zα2 domain in ZBP1. Amino acid residues (aa) present at the N terminus (red) and C terminus (green) of the Zα2 domain that are in sequence after deletion of this domain are represented. B, immunoblot analysis of ZBP1 after mock or LPS stimulation of WT and Zbp1ΔZα2/ΔZα2 BMDMs. ZBP1-iso-2, ZBP1 isoform 2.
