Figure 2.
The Zα2 domain of ZBP1 is critical for triggering IAV-induced NLRP3 inflammasome activation and PANoptosis. A, immunoblot analysis of ZBP1, CASP1, GSDMD, IAV-NS1, and GAPDH in WT and Zbp1ΔZα2/ΔZα2 BMDMs infected with IAV (mouse-adapted, influenza A/Puerto Rico/8/34 (PR8; H1N1)). B and C, Cell death as measured by the number of SYTOX Green+ cells. BMDMs were infected with IAV, and cell death was monitored at regular intervals. ****, p < 0.0001 (one-way analysis of variance) for B; ****, p < 0.0001 (unpaired two-sided t test) for C. Data are represented as mean ± S.E. (B and C) and are representative of three independent experiments.