Figure 3.

The Z-nucleic acid–binding Zα2 domain of ZBP1 is essential for embryonic development and acts as an activator of RIPK1-RHIM mutation-induced perinatal lethality in mice. A, the number of offspring of each genotype generated from intercrossing Ripk1^+/mRHIM Zbp1^+/ΔZα2 (heterozygote) parents. B, the number of offspring of each genotype generated from intercrossing Ripk1^+/mRHIM Zbp1^{ΔZα2/ΔZα2} parents. In A and B, the numbers of Ripk1^mRHIM/mRHIM mice rescued from perinatal lethality are shown in red. C, Kaplan–Meier plot showing survival of Ripk1^mRHIM/mRHIM Zbp1^{ΔZα2/ΔZα2} mice and Ripk1^mRHIM/mRHIM Zbp1^−/− mice compared with WT Ripk1^+/+ Zbp1^+/+ mice.