Figure 4.
Increasing GPR68 copy number potentiates Gα coupling and signaling strength. A, Gα coupling as a function of GPR68 copy number in the 10 four-padded GPCR reporter strains. Experiments were performed at pH 6.0 to activate GPR68. The data are reported as RFU at an A600 nm of 1.0 and instrument gain of 1200 for strains expressing one (X-2), two (X-2, XII-5), three (X-2, XII-5, X-3), and four (X-2, XII-5, X-3, XI-2) copies of GPR68. B, Gα coupling as a function of GPR68 copy number in each of the 10 four-padded GPCR reporter strains shown in A. The error bars in A and B represent the S.D. of four biological replicates.