Figure 2.

Suramin has no significant impact on abundance or location of selected enzymes of the glycosomal pathway. A and B, steady-state levels of PFK, PGK, and PYK were analyzed by immunoblotting at 0 (CON), 3, or 4 days post-suramin treatment (1× EC₅₀). β-tubulin was used as loading control. Graphs represent the mean of three independent experiments, with the S.E. indicated. C, cells were treated with 1× EC₅₀ suramin for 0 or 4 days, and the subcellular localizations of PFK, PGK, and PYK were detected using antibodies specific to each protein. Cells were stained with 4′,6-diamidino-2-phenylindole to visualize nuclear and mitochondrial DNA. Scale bar, 2 μm.