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. 2020 Apr 24;295(24):8302–8324. doi: 10.1074/jbc.RA119.012372

Table 2.

Summary of human HspA1A (hHsp70) mutants used in this study

Name of protein Description
WT hHsp70 hHsp70 (HspA1A/B)
hHsp70-SSSCC hHsp70 C17S/C267S/C306S
hHsp70-CCCSS hHsp70 C574S/C603S
hHsp70-CSSCC hHsp70 C267S/C306S
hHsp70-CSSAA hHsp70 C267S/C306S/C574A/C603A
hHsp70-CSSAS hHsp70 C267S/C306S/C574A/C603S
hHsp70-CSSSS hHsp70 C267S/C306S/C574S/C603S
hHsp70-CCCQQ hHsp70 C574Q/C603Q, mimics glutathionylation of CTD
hHsp70-CSSCA hHsp70 C267S/C306S/C603A
hHsp70-CSSAC hHsp70 C267S/C306S/C574A
WT hHsc70 hHsc70 (HspA8)
SBDα(537–610) or SBDα(537–610)-CC hHsp70 Δ1–536/Δ611–641, SBDα of hHsp70 consisting of remote part of α-helix B and α-helices C and D
SBDα(537–610)-AA hHsp70 Δ1–536/Δ611–641/C574A/C603A
SBDα(537–610)-AS hHsp70 Δ1–536/Δ611–641/C574A/C603S
SBDα(537–610)-SS hHsp70 Δ1–536/Δ611–641/C574S/C603S
SBDα(537–610)-QQ hHsp70 Δ1–536/Δ611–641/C574Q/C603Q
hHsp70(385–641) hHsp70 Δ1–384, SBD of hHsp70
hHsp70 T204A hHsp70 mutant that binds but does not hydrolyze ATP