Figure 5.

HAND2‐AS1 represses the tumorigenesis of cervical cancer in vivo through C16orf74 and E2F4. A‐C, the tumour volume and weight in nude mice bearing the HAND2‐AS1 overexpressed cells upon E2F4 knock‐down or C16orf74 overexpression; *P < .05. D, The mRNA expression of C16orf74 in tumour tissues was detected by RT‐qPCR; *P < .05. E, The protein expression of N‐cadherin, vimentin, MMP‐2 and MMP‐9 in tumour tissues of mice examined by Western blot analysis; *P < .05. The result data were measurement data and were expressed as mean ± standard deviation. Data that conformed to normal distribution were compared by unpaired t test between two groups. The repeated‐measures ANOVA was applied for comparing the data among multiple groups at different time‐points, and the Bonferroni's post hoc test was conducted. n = 12. RT‐qPCR, reverse transcription quantitative‐polymerase chain reaction; HAND2‐AS1, heart‐ and neural crest derivative‐expressed protein 2‐AS1; oe, overexpression; NC, negative control; MMP, matrix metallopeptidase; ANOVA, analysis of variance; C16orf74, chromosome 16 open reading frame 74