FIGURE 6.

Protein expression of osteogenic signalling pathway molecules expressed by VEC and VIC from 3D constructs, cultured in NG or HG conditions. A and B, Soluble BMP‐2 and TGF‐β released in the conditioned media by valvular cells from 3D construct exposed to NG or HG conditions, as determined by ELISA assay. n = 3, *P < .05. C‐F, Protein expression of BMP‐2, pSMAD1/5/8/9, pSMAD2/3 and Runx‐2 in VIC and VEC as determined by Western blot. n = 3, *P < .05**P < .01 high glucose vs normal glucose