FIGURE 2.

LncRNA TBX5‐AS1:2 affected proliferation of HEK293T cells. A, Three shRNAs targeting the non‐overlapping regions of lncRNA TBX5‐AS1:2 were designed to knock‐down lncRNA TBX5‐AS1:2 expression. The shRNA2 with the greatest interference efficiency was used to establish stable cell lines. B, LncRNA TBX5‐AS1:2 was successfully overexpressed. C and D Positive regulation of cell proliferation by lncRNA TBX5‐AS1:2 was revealed by CCK8 assays. E and F, LncRNA TBX5‐AS1:2 had no effect on cell apoptosis according to flow cytometry analysis. Values are mean ± SEM, n = 3, ***P < .0001. HEK293T, human embryonic kidney 293; lncRNAs, long non‐coding RNAs; shRNAs, short hairpin RNAs