Figure 4.

HOTTIP regulates miR‐148a‐3p by acting as a ceRNA. A, A putative binding site between HOTTIP and miR‐148a‐3p by DIANA TOOLS. B, C, D, Luciferase reporter assay in the HEK‐293T, MCF7 and T47D cell lines cotransfected with WT and MUT type HOTTIP reporters and miR‐148a‐3p mimics. E, F, qRT‐PCR was performed to test the expression of miR‐148a‐3p in the sh‐HOTTIP‐transfected sphere cells of MCF7 and T47D, and the OE‐HOTTIP‐transfected parental cells. Data are presented as mean ± SD. **P < .01, ***P < .001 compared to control or shControl group