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. 2020 Apr 19;24(11):6242–6252. doi: 10.1111/jcmm.15261

Figure 5.

Figure 5

HOTTIP modulates the stemness of BCSCs via miR‐148a‐3p/WNT1 signaling. A, C, The effects of miR‐148a‐3p inhibitor on the self‐renewal capacity of the shHOTTIP‐transfected MCF7 and T47D sphere cells by sphere formation assays. B, D, The effects of miR‐148a‐3p mimic on the self‐renewal capacity of the OE‐HOTTIP‐transfected MCF7 and T47D parental cells by soft‐agar colony formation. E, The effects of miR‐148a‐3p inhibitor on the WNT1 protein levels of the shHOTTIP‐transfected MCF7 and T47D sphere cells by Western blot assay. F, The effects of miR‐148a‐3p mimic on the WNT1 protein levels of the OE‐HOTTIP‐transfected MCF7 and T47D parental cells. G, The correlation between HOTTIP and miR‐148a‐3p was analysed by Spearman's correlation analysis. H, The relationship of HOTTIP and WNT1 expression was analysed by Spearman's correlation analysis. I, GEO dataset revealed a significant positive correlation between HOTTIP and WNT1 expression. Data are presented as mean ± SD. *P < .05, **P < .01, compare with Control or shControl group