Figure 1.

Luspatercept inhibits Smad2/3 activation by activin B, GDF8 and GDF11 in mouse erythroleukemic cells. Western blot analysis showing effect of GDF11 (100 ng/mL), activin B (10 ng/mL), GDF8 (100 ng/mL) and activin A (10 ng/mL) alone or in combination with luspatercept (1 μg/mL) on pSmad2/3 levels in MEL cells pretreated with 2% DMSO (control) to induce differentiation. Control lane represents untreated MEL cells, and HT‐1080 cell extracts treated with hTGFβ3 serve as the positive control