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. 2020 Apr 29;24(11):6162–6177. doi: 10.1111/jcmm.15243

Figure 5.

Figure 5

Luspatercept increases GATA‐1 nuclear availability in MEL cells. A, Immunofluorescence microscopy showing effect of GDF11 (100 ng/mL, 24 h) alone or in combination with luspatercept (1 µg/mL, 24 h) on cellular levels of GATA‐1 in DMSO‐pretreated MEL cells (control). Images depict GATA‐1 (green/AF488), HSP70 (red/AF594) and DAPI‐labelled nuclei (blue). Scale bar 10 μm. B, GATA‐1 levels determined by mean fluorescence intensity. Data are means ± SEM (n = 4 representative images per group), *P < .05 vs. GDF11. C, Western blot analysis of nuclear extracts from DMSO‐pretreated MEL cells (control) treated additionally with GDF11 (100 ng/mL) or combined GDF11 (100 ng/mL) and luspatercept (1 µg/mL). Lamin B1 was used as a nuclear loading control