FIGURE 1.

CGRP inhibited oxidative stress in Ang II‐induced VSMCs. Vascular smooth muscle cells (VSMCs), stimulated with CGRP (10⁻⁶‐10⁻⁸ mol/L) for 60 min, CGRP_8‐37 (3 × 10⁻⁵ mol/L) for 30 min or apocynin (10⁻⁶ mol/L) for 2 h, were treated with Ang II (10⁻⁷ mol/L) for 30 min. A, Quantification of intracellular reactive oxygen species (ROS) levels. ROS levels were measured using DCFH‐DA as described in method. B, Quantitation of the intracellular NADP/NADPH ratio. C, Western blot analysis of membrane and cytoplasmic fractions of p47phox in VSMCs. D, Quantitative real‐time PCR analysis of mRNA levels of p47phox in VSMCs. Bar graphs show mean ± SEM values from three independent experiments. *P < .05, **P < .01 and ***P < .001 vs Ang II. ^# P < .05 vs control