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. 2020 May 5;24(11):6426–6437. doi: 10.1111/jcmm.15288

FIGURE 5.

FIGURE 5

Src/STAT3 signalling pathway is involved in the antioxidant activity of CGRP. VSMCs, stimulated with CGRP (10−7 mol/L) for 60 min or CGRP8‐37 (3 × 10−5 mol/L) for 30 min, were treated with Ang II (10−7 mol/L) for 30 min. In some experiments, PP2 (10−5 mol/L) or niclosamide (10−5 mol/L) was applied 20 min before CGRP pre‐treatment. VSMCs were transfected with Src ORF cDNA clones (Src) or STAT3 ORF cDNA clones (STAT3) and incubated for 24 h. Increased Src (A) and STAT3 (B) protein levels in VSMCs were evaluated via Western blot analysis after transfection with Src or STAT3 ORF cDNA clones, respectively. (C), Quantification of intracellular ROS levels in VSMCs via DCFH‐DA. Src group, VSMCs were transfected with Src ORF cDNA clones; STAT3 group, VSMCs were transfected with STAT3 ORF cDNA clones; and control group, VSMCs were transfected with blank vector. Bar graphs show mean ± SEM values from three independent experiments. *P < .05, **P < .01 and ***P < .001 vs Ang II. # P < .05 vs control. + P < .05 vs CGRP + Ang II