FIGURE 5.

The expression and release of Klotho protein from the rat heart tissue under I/R injury. A, 65 kDa bands of Klotho protein in the rat heart tissue. Qualitative examination by Western blot. B, The concentration of Klotho protein in the rat heart tissue by ELISA. Klotho protein concentration in tissue homogenates was normalized to total protein concentration. C, Klotho content in coronary effluents (collected at the beginning of reperfusion) by Western blot. The content of Klotho protein in coronary effluents was expressed as AU and normalized to CF. D, Correlation between Klotho content in coronary effluents and cardiac mechanical function and E, LDH level. AU, arbitrary units; CF, coronary flow; LDH, lactate dehydrogenase; mU/mL, milli international enzyme units per millilitre; *P < .05 vs aerobic control; mean ± SEM; n = 4‐8