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An influence of Klotho protein on cell viability. The number of death cells in the presence and absence of recombinant Klotho protein (1 μg/mL) by luminescent cytotoxicity assay. The data were normalized to cell confluence and expressed in RLU. The viability of myocytes was based on the number of death cells. I/R, ischaemia/reperfusion; RLU, relative light units; *P < .05 vs aerobic control; # P < .05 vs aerobic control + Klotho; $ P < .05 vs I/R; mean ± SEM; n = 8
