FIGURE 5.

Loganin relieved AGE‐induced GC‐2 cell apoptosis detected by AO/EB staining and FCM. A, AO/EB staining showed that apoptotic cells emitted orange fluorescence and normal cells emitted green fluorescence (200 × magnification). B, FCM of GC‐2 cells treated with loganin. C, Statistical chart of AO/EB staining. D, Statistical chart of FCM. (E and F) Western blot analyses of Bax and Bcl‐2 protein expressions in GC‐2 cells. Bax/Bcl‐2 ratio was calculated and analysed. Bars represent mean ± SD, n = 3. Significance: ^## P < .01 vs control group; **P < .01 vs AGEs group