FIGURE 6.

miR‐7212‐5p suppresses osteoblast differentiation. A, Relative expression of miR‐7212‐5p in MC3T3‐E1 cells after transfection with PBS, antagomiR‐NC, antagomiR‐7212‐5p, agomiR‐NC or agomiR‐7212‐5p. B, Relative levels of Collagen I, ALP, OCN and Runx2 in MC3T3‐E1 cells were quantified using qRT‐PCR. C, ALP staining of MC3T3‐E1 cells after transfection with either PBS, antagomiR‐NC, antagomiR‐7212‐5p, agomiR‐NC or agomiR‐7212‐5p. D, Quantification of the absorbance at 405 nm in (C) groups. E, Alizarin red staining of MC3T3‐E1 cells after 21 d following transfection with PBS, antagomiR‐NC, antagomiR‐7212‐5p, agomiR‐NC or agomiR‐7212‐5p. F, Quantification of the absorbance at 570 nm in (E) groups. The data are expressed as mean ± SD. Scale bar = 50 μm. All experiments were performed in triplicates. ****P < .001