Fig. 2.

(A) Calculation of the maximum specific binding (B_max) and affinity (K_d) of anti-ICAM-1 antibody using flow cytometry, shows a 1.8-fold increase in B_max value when comparing mock-treated to irradiated PSN-1 cells. (B) iTLC of ¹¹¹In-labelled anti-ICAM-1 antibody, showing efficient conjugation of >95%. (C) The binding affinity of the anti-ICAM-1 DTPA/¹¹¹In radio-conjugate ([¹¹¹In]In-anti-ICAM-1), at 24 h time-point was determined using an in vitro saturation binding methodology. A negative control [¹¹¹In]In-mIgG2a was also characterized.