Figure 1.

VSV_AD17 as B Cell Probe for HIV-1 bNAb Isolation

(A) Schematic VSV_AD17 constructs. Abbreviations are as follows: mNG, monomeric NeonGreen; TM, transmembrane; CT, cytoplasmic tail.

(B) Fluorescence-activated cell sorting (FACS) of pre-sorted B cells from donor M4008 for single IgG⁺ memory B cells bound to VSV_AD17-PE. Abbreviations are as follows: SSC-A, side-scatter area; FSC-A, forward-scatter area.

(C) FACS of pre-sorted B cells from donor M1214 for single IgM⁻CD27⁺ memory B cells bound to VSV_AD17-mNG.

(D) Neutralization breadth and potency of three bNAb clones from M4008 and M1214 evaluated on a total of 120 cross-clade HIV-1 Env strains. The dendrograms show the Env protein sequence distance, with clades, including circulating recombinant forms (CRFs), indicated. The neutralization potency is color-coded for the branch of each Env tested. The data under the dendrograms show the number and percent of viruses neutralized with an IC₅₀ < 50 μg/mL and the geometric mean IC₅₀ value for viruses neutralized with an IC₅₀ < 50 μg/mL.