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. 2020 Jun 10;27(6):963–975.e5. doi: 10.1016/j.chom.2020.03.024

Figure 4.

Figure 4

Epitope Mapping of HIV-1 bNAbs

(A) Epitope mapping of bNAbs by comparison of neutralization profiles of each bNAb on the HIV-1 strain JR-FL, as WT, treated with Kifunensine (Kif), or with indicated point mutations. Neutralization was performed in duplicate wells, and the data shown are means with SEM.

(B) Epitope mapping of bNAbs by standard and competition ELISAs. The competition ELISAs were performed with a single concentration of biotinylated reagent (bNAbs or CD4-Ig, as indicated) binding to JR-FL gp120 or CH505 SOSIP. The unlabeled competing mAbs or CD4-Ig were titrated into the ELISAs at increasing concentrations to evaluate the effect on biotinylated reagent binding.