Table 1.

Tests performed for the image manipulation selection

Test on BSA	1	2	3	4	5	6	7
Brightness and contrast correction	Auto	Auto	Auto	N. A.	N. A.	N. A.	Auto
Rolling ball radius [pixel]	N. A.	50	150	50	150	250	250
Oversaturation over black [%]	0.19	0.00	0.04	0.00	0.00	0.00	0.00
Oversaturation over white [%]	0.01	20.05	10.8	18.34	10.33	6.56	6.29
Flattened peaks at high protein load	Yes	Yes	Yes	No	No	No	Yes
R 2 for grayscale profile [/]	.822	.852	.896	.935	.917	.872	.879
p‐Value for grayscale profile [/]	.013	.009	.004	.002	.003	.006	.006
R 2 for OD profile [/]	.993	.950	.974	.995	.992	.984	.975
p‐Value for OD profile [/]	2.28E−04	9.43E−04	2.54E−04	7.90E−06	2.44E−05	9.57E−05	2.29E−04

Brightness and contrast adjustments were made with the automatic function. The background subtraction was made using the rolling ball radius at the given size. Flattened peaks found in both optical density and grayscale profiles. R ² and p‐values were calculated for the BSA.

Abbreviation: N. A.: not applied.