Table 2.

Regression analysis for bovine serum albumin (BSA), carbonic anhydrase (CA), and ovalbumin (OV) and molecular weight markers

Protein	BSA	CA	OV	100 kDa	50 kDa	20 kDa
Slope [(pixel*AU)/ng]	4.032	3.749	2.375	2.058	3.193	7.524
σ [(pixel*AU)/ng]	0.257	0.185	0.046	0.176	0.064	0.380
R 2 [/]	.984	.99	.998	.986	.999	.995
p‐Value [/]	9.57E−05	3.49E−05	8.62E−07	7.26E−03	3.98E−04	2.54E−03

Equation was established as A = slope*P. A: peak area, P: protein load.