Table 2.

RT-PCR primers used to target MHC genes and associated NATs

Target Description	Primer Sequence
I mRNA
Forward	5′-GGTGCGGGAGCTGGAGAATG-3′
Reverse	5′-GGGGCTTTGCTGGCACCTC-3′
I pre-mRNA
Forward	5′-CATCACGCGTTTATCCAGAAGCTCATTAT-3′
Reverse	5′-TGCCAGGTTATCTACACCCAAAATCCAC-3′
IIa mRNA
Forward	5′-GGGTACGGGAGCTGGAAGGAGAGG-3′
Reverse	5′-TTACAGAGGGAAATGACCAAAGATG-3′
IIa pre-mRNA
Forward	5′-GGTTGCCAAAATAGGAATGTGAGATAC-3′
Reverse	5′-ATTTAGCTAGATTGGTGTTGGATTGTTC-3′
aII NAT
Forward	5′-ATGATAATCCCTAGATGTTGCTTCTCCATAGAT-3′
Reverse	5′-CTCTTCACCCGCAGTTTGTTCACC-3′
IIx mRNA
Forward	5′-CAGGACACCAGCGCCCATCT-3′
Reverse	5′-TTTCTTTGGTCACCTTTCAGCAGTT-3′
IIx pre-mRNA
Forward	5′-TATCCTGCCACCATTATAGCTTTATGTTC-3′
Reverse	5′-CCACCTCCCTGGCTTGGTTGAG-3′
xII NAT
Forward	5′-TTCCTCTCCCAGCCAGGGTCCTTA-3′
Reverse	5′-AGGCAGAGTCTAATCAGCTCCAGGTGTTT-3′
IIb mRNA
Forward	5′-AAGAATATTCTCAGGCTGCAGGACTTG-3′
Reverse	5′-ACATTTCGTGCATTTCTTTGGTCACAT-3′
IIb pre-mRNA
Forward	5′-TGTGTTGTTAATTCAAGTTGCCTGGTGTAA-3′
Reverse	5′-TGTCATCAGCATTAACTTTCCCTCAACTTA-3′
bII NAT
Forward	5′-AACATTCTACAACCCCCAGCACTCA-3′
Reverse	5′-AGCAAGGCATCCCACAAACTCG-3′

MHC, myosin heavy chain; NAT, natural antisense RNA transcript.