Table 3.

RT-PCR primers used to target sense and antisense RNA expression in the MHC genes

Target Description	Primer Sequence
Emb-IIa IG1
Forward	5′-AGCTGCTATGGATGCTCGTC-3′
Reverse	5′-CATGCCCAGGAACAGGTGAT-3′
Emb-IIa IG2
Forward	5′-AGGCCTACGTATTGTGCAACT-3′
Reverse	5′-ACGCTGACCTTTTTGCTTGT-3′
Emb-IIa IG3
Forward	5′-TCAGATGCCCTGGGTTTCTTT-3′
Reverse	5′-GCTGGTATGCAGCCAAAAGT-3′
IIa-IIx IG
Forward	5′-GCTCCTGCTTCCCCTAACA-3′
Reverse	5′-TGAGTGGTCCAGATTGCTATGA-3′
IIx-IIb IG1
Forward	5′-TATGCACCTCCACTTATTTTCTATTG-3′
Reverse	5′-TATGCCCATTTATTTATCGTTTTT-3′
IIx-IIb IG2
Forward	5′-GATTTTGTTTTTAACGCCTGATGACCTATT-3′
Reverse	5′-CTCCCTGTGGATTGAAAAGATGGATG-3′
IIb-Neo IG1
Forward	5′-ATCATAGCCATTGCCATAGTGT-3′
Reverse	5′-AATTGCCCAGTGCTTTTGA-3′
IIb-Neo IG2
Forward	5′-TTGTCCTTGCTCTTCTTCGCT-3′
Reverse	5′-AGCAGAAGGGTCATTGGATACG-3′
IIb-Neo IG3
Forward	5′-ACTAACATGGCAAATGATCCAAGAGTGTAG-3′
Reverse	5′-ACCCGTTTAGTTTGTAATATTGCAGACCTT-3′
Neo
Forward	5′-CAGCCTGGATGATCTACGTGAGTGTCT-3′
Reverse	5′-ATAACATAAAGAGGCTCGGAAGAGGTCTG-3′