Fig. 1.

Pulldown of ubiquitinated proteins and the α-subunit of the epithelial Na⁺ channel (αENaC) from the mouse kidney. Lanes of the Western blot were loaded with 50 μg of whole kidney extract (input to the affinity beads), 45 μL of eluates from the ubiquitin-affinity beads, or 45 μL eluates from the control-affinity beads. A: blots were stained using anti-ubiquitin horseradish peroxidase. B: a similar blot stained with anti-αENaC antibody. Mice were fed either a control diet or a low-Na⁺ diet for 1 wk. Results are representative of at least 3 experiments with mouse and rat kidneys.