Fig. 3.

Pulldown of the ubiquitinated γ-subunit of the epithelial Na⁺ channel (γENaC) from the mouse kidney. Lanes of the Western blot were loaded with 50 μg of whole kidney extract (input to the affinity beads), 45 μL of eluates from the ubiquitin-affinity beads, or 45 μL eluates from the control-affinity beads. Blots were stained using anti-γENaC antibody. Animals were fed either a control diet or a low-Na⁺ diet for 1 wk. Results are representative of at least 3 experiments with mouse and rat kidneys.