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Pulldown of the ubiquitinated γ-subunit of the epithelial Na+ channel (γENaC) from mouse kidneys analyzed under nonreducing conditions. Lanes of the Western blot were loaded with 15 μg of whole kidney (homogenate) or 40 μL of eluates from the ubiquitin-affinity beads (pulldown) from three different animals fed a low-Na+ diet for 1 wk. Blots were stained using anti-γENaC antibody. Results are representative of 3 experiments from rats and mice.
