Fig. 1.

ELABELA (ELA) expression in the adult mouse kidney. A: RNAscope with mApela (m, Mus musculus) probe in the mouse kidney. Clear and punctate Apela labeling is present in the collecting ducts (CDs), especially in the outer medulla CDs and inner medulla CDs. B: immunofluorescence staining of ELA in the mouse kidney. To validate the specificity of the labeling of renal ELA in mice, the competition with the immunizing peptide in the consecutive sections from the same tissue samples was performed, and the consecutive sections were stained with ELA antibody preincubated with or without its immunizing peptides. Male mice were used in these studies. AQP2, aquaporin 2.