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. 2020 Mar 16;318(5):F1122–F1135. doi: 10.1152/ajprenal.00606.2019

Table 1.

Sequences of oligonucleotides used for quantitative RT-PCR

Gene Forward Primer (5′→3′) Reverse Primer (5′→3′) Product Size, bp
R PRR ATCCTTGAGACGAAACAAGA AGCCAGTCATAATCCACAGT 109
R Renin GATCACCATGAAGGGGGTCTCTGT GTTCCTGAAGGGATTCTTTTGCAC 274
R AGT AGCATCCTCCTTGAACTCCA TGATTTTTGCCCAGGATAGC 244
R ACE GAGCCATCCTTCCCTTTTTC CCACATGTTCCCTAGCAGGT 266
R AT1R TGAGCACGCTTTCTTACC CTGCTTAGATCCTGAGGC 189
R AT2R CAATCTGGCTGTGGCTGACTT TGCACATCACAGGTCCAAAGA 101
R ACE2 GAATGCGACCATCAAGCG CAAGCCCAGAGCCTACGA 228
R COX-2 TCCTCAGAAGAACCTTTTCC GGAGTCTGGAACATTGTGAA 265
R TNF-α AGTGACAAGCCCGTAGCC GGGTGAGGAGCACGTAGTC 205
R IL-1β CGACAGTGAGGAGAATGACC TCCACGGGCAAGACATAG 206
R IL-2 AGCGTGTGTTGGATTTGACTC ATGATGCTTTGACAGATGGCTA 190
R IL-6 GCCAGAGTCATTCAGAGCAATA GTTGGATGGTCTTGGTCCTTAG 160
R IL-17A GGGAAGTTGGACCACCACCT TTCTCCACCCGGAAAGTGAA 101
R IFN-γ AGGCCATCAGCAACAACATAAGTG GACAGCTTTGTGCTGGATCTGTG 140
R Apela ATTCTCGAGTGCCCTTCCCATG TCCGAAAAGCCATCCAAGGTAC 174
R Apln GACCCATGCCTTTCTAAAGCAG TAACAGGTGCAAGATGAGAGCC 131
R Aplnr CAGGGTGCTTGCTGAGTTGCTG GGAAAACCAGAATGTAGATGGC 194
R TGF-β1 CACGATCATGTTGGACAACTGCTCC CTTCAGCTCCACAGAGAAGAACTGC 298
R Fibronectin AGACCATACCTGCCGAATGTAG GAGAGCTTCTTGTCCTGTAGAG 129
R Collagen1a ACGCATGGCCAAGAAGACATCCC TTGCATAGCACGCCATCGCACAC 143
R PAI-1 TGGTGAACGCCCTCTATTTC GAGGGGCACATCTTTTTCAA 248
R NGAL TCTGGGCCTCAAGGATAACAAC AGACAGGTGGGACCTGACCA 127
R TIMP-1 GACCACCTTATACCAGCGTT GTCACTCTCCAGTTTGCAAG 334
R KIM-1 CTCCAGGAAGCCGAGCAAAC AAGCACTGGGTACAGATCCAAA 124
R MCP-1 GCCCCACTCACCTGCTGCTACT CCTGCTGCTGGTGATTCTCTTGT 87
R ICAM-1 TACAAGTGCCGTGCCTTTAG CATGGTACAGCACTGTCAGGT 70
R VCAM-1 GCTACATCCACACTGACGCT CAGGGAATGAGTAGACCTCCA 124
R GAPDH GTCTTCACTACCATGGAGAAGG TCATGGATGACCTTGGCCAG 197
M PRR TCTCCGAACTGCAAGTGCTA CTGCAAACTTTTGGAGAGCA 195
M Apela TTTGCAGAGACTTCCCGCTT GCTCACCCCACATCCTATGG 93
M Apln GCTGCTGCTGCTCTGGCTCT GGGGGCGCTGTCTGCGAAAT 176
M Aplnr GCCTGTCATGGTGTTCCG CTCAATGCGCTCCTTTCGG 229
M GAPDH AGGTCGGTGTGAACGGATTTG TGTAGACCATGTAGTTGAGGTCA 123

M, Mus musculus; R, Rattus norvegicus.